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Gustav Bach introduced the cigar band in the 19th century to prevent cigars from staining gloves

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Then, they were incubated with primary antibodies at 4 C for overnight and stained with FITC- (A0562, beyotime) and Coralite594-conjugated secondary antibody (SA00013-4, Proteintech) at room temperature for 1 h, after which nuclear were stained with DAPI (F6057, sigma)